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rna  (AMS Biotechnology)


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    Structured Review

    AMS Biotechnology rna
    Rna, supplied by AMS Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 559 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rna+stat+60/RNA+STAT-60/pm41991686-394-9-16
    Average 96 stars, based on 559 article reviews
    rna - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Purification:

    Article Title: Programmable kinetic barcoding for multiplexed RNA detection with Cas13a.
    Article Snippet: SARS-CoV-2 N gene, S gene (wild type (WT)) and S gene with D614G mutation were transcribed off a single-stranded DNA oligonucleotide template (IDT) using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) following the manufacturer’s recommendations. .. Template DNA was removed by addition of DNase I (NEB), and IVT RNA was subsequently purified using RNA STAT-60 (AMSBIO) and the Direct-Zol RNA MiniPrep Kit (Zymo Research). .. RNA concentration was quantified by Nanodrop, and copy number was calculated using transcript length and concentration.

    Isolation:

    Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease.
    Article Snippet: .. For all human PBMC and mouse BMDM cell-culture experiments, RNA was isolated using RNA STAT 60 (Amsbio) as per the manufacturer’s protocol. .. Approximately 2,000 ng of RNA was converted to complementary DNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease
    Article Snippet: .. For all human PBMC and mouse BMDM cell-culture experiments, RNA was isolated using RNA STAT 60 (Amsbio) as per the manufacturer’s protocol. .. Approximately 2,000 ng of RNA was converted to complementary DNA using a High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems).

    Sequencing:

    Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease
    Article Snippet: .. Mouse BMDM-derived and human PBMC-derived macrophage cultures were lysed with RNA STAT 60 (Amsbio) and ~50 ng per μl of RNA was submitted to the UCLA’s Technology Center for Genomics and Bioinformatics (TCGB) core for library preparation and 2 × 100 paired-end sequencing. .. Roughly 100 million high-quality reads were sequenced per sample using NovaSeq X Plus (Illumina).

    Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease.
    Article Snippet: .. Mouse BMDM-derived and human PBMC-derived macrophage cultures were lysed with RNA STAT 60 (Amsbio) and ~50 ng per μl of RNA was submitted to the UCLA’s Technology Center for Genomics and Bioinformatics (TCGB) core for library preparation and 2 × 100 paired-end sequencing. .. Roughly 100 million high-quality reads were sequenced per sample using NovaSeq X Plus (Illumina).



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    a , Illustration of the in vitro model system using exogenous DNA damage with irradiation and Doxo <t>on</t> <t>PBMC-derived</t> macrophages from male and female human donors. b , Mean ± s.e. gene expression (RT–qPCR) normalized to control. n = 9 donors (mixed male and female) for control and Sen(IR) conditions. n = 8 for Sen(Doxo) conditions. P value from Tukey’s test post repeated measures ANOVA analysis. c , SDS–PAGE gels and immunostaining (western blot) for p21 and p16 in response to 10 days post DNA damage. ImageJ quantification for band intensity is shown to the right and represents relative intensity over the loading control. d , SA-β-gal images of human senescent macrophages. e , Click-iT EdU labeling of cells to assess proliferation dynamics. Axes represent the percentage of cells positive for the stain relative to the parental gate, as detected by flow cytometry. The bar plot represents the mean ± s.e. of n = 3 independent donors. P value derived from unpaired one-sided t -test. f , PCA on bulk RNA-seq samples from 10 days post DNA damage for three blood donors. g , Heatmap projection of all statistically significant DEGs compared to control conditions. h , KEGG pathway enrichment analysis for the top ten pathways downregulated in human senescent macrophages. i , KEGG pathway enrichment analysis for the top ten pathways upregulated in human senescent macrophages. j , Model illustration of running MSen score on publicly accessible single-cell dataset of human liver biopsy samples from five people with liver cirrhosis and five with a healthy liver. k , UMAP projection of all annotated CD45 + cell types and colored by human MSen Seurat score. l , UMAP projection of CD68 + cells (macrophages) in healthy and cirrhotic tissue samples. Color denotes human MSen Seurat score. m , Distribution of MSen Seurat score across resident <t>macrophage</t> (Kupffer cells) and non-resident monocyte and macrophage populations in healthy and cirrhotic conditions. P value represents the results of a two-sided t -test. n , Distribution of human MSen score across all CD45 + cell types in the dataset. ALD, alcohol-related liver disease; F, female; KC, Kupffer cell; IgA, immunoglobulin A; IL-C, innate lymphoid cell; M, male; MoMac, monocyte-derived macrophage; Mø, macrophage; PBC, primary biliary cholangitis; pDC, plasmacytoid dendritic cell. Panels a and j created in BioRender; Salladay-Perez, I. https://biorender.com/6umuckd (2026).
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    Image Search Results


    a , Illustration of the in vitro model system using exogenous DNA damage with irradiation and Doxo on PBMC-derived macrophages from male and female human donors. b , Mean ± s.e. gene expression (RT–qPCR) normalized to control. n = 9 donors (mixed male and female) for control and Sen(IR) conditions. n = 8 for Sen(Doxo) conditions. P value from Tukey’s test post repeated measures ANOVA analysis. c , SDS–PAGE gels and immunostaining (western blot) for p21 and p16 in response to 10 days post DNA damage. ImageJ quantification for band intensity is shown to the right and represents relative intensity over the loading control. d , SA-β-gal images of human senescent macrophages. e , Click-iT EdU labeling of cells to assess proliferation dynamics. Axes represent the percentage of cells positive for the stain relative to the parental gate, as detected by flow cytometry. The bar plot represents the mean ± s.e. of n = 3 independent donors. P value derived from unpaired one-sided t -test. f , PCA on bulk RNA-seq samples from 10 days post DNA damage for three blood donors. g , Heatmap projection of all statistically significant DEGs compared to control conditions. h , KEGG pathway enrichment analysis for the top ten pathways downregulated in human senescent macrophages. i , KEGG pathway enrichment analysis for the top ten pathways upregulated in human senescent macrophages. j , Model illustration of running MSen score on publicly accessible single-cell dataset of human liver biopsy samples from five people with liver cirrhosis and five with a healthy liver. k , UMAP projection of all annotated CD45 + cell types and colored by human MSen Seurat score. l , UMAP projection of CD68 + cells (macrophages) in healthy and cirrhotic tissue samples. Color denotes human MSen Seurat score. m , Distribution of MSen Seurat score across resident macrophage (Kupffer cells) and non-resident monocyte and macrophage populations in healthy and cirrhotic conditions. P value represents the results of a two-sided t -test. n , Distribution of human MSen score across all CD45 + cell types in the dataset. ALD, alcohol-related liver disease; F, female; KC, Kupffer cell; IgA, immunoglobulin A; IL-C, innate lymphoid cell; M, male; MoMac, monocyte-derived macrophage; Mø, macrophage; PBC, primary biliary cholangitis; pDC, plasmacytoid dendritic cell. Panels a and j created in BioRender; Salladay-Perez, I. https://biorender.com/6umuckd (2026).

    Journal: Nature Aging

    Article Title: p21 + TREM2 + senescent macrophages fuel inflammaging and metabolic dysfunction-associated steatotic liver disease

    doi: 10.1038/s43587-026-01101-6

    Figure Lengend Snippet: a , Illustration of the in vitro model system using exogenous DNA damage with irradiation and Doxo on PBMC-derived macrophages from male and female human donors. b , Mean ± s.e. gene expression (RT–qPCR) normalized to control. n = 9 donors (mixed male and female) for control and Sen(IR) conditions. n = 8 for Sen(Doxo) conditions. P value from Tukey’s test post repeated measures ANOVA analysis. c , SDS–PAGE gels and immunostaining (western blot) for p21 and p16 in response to 10 days post DNA damage. ImageJ quantification for band intensity is shown to the right and represents relative intensity over the loading control. d , SA-β-gal images of human senescent macrophages. e , Click-iT EdU labeling of cells to assess proliferation dynamics. Axes represent the percentage of cells positive for the stain relative to the parental gate, as detected by flow cytometry. The bar plot represents the mean ± s.e. of n = 3 independent donors. P value derived from unpaired one-sided t -test. f , PCA on bulk RNA-seq samples from 10 days post DNA damage for three blood donors. g , Heatmap projection of all statistically significant DEGs compared to control conditions. h , KEGG pathway enrichment analysis for the top ten pathways downregulated in human senescent macrophages. i , KEGG pathway enrichment analysis for the top ten pathways upregulated in human senescent macrophages. j , Model illustration of running MSen score on publicly accessible single-cell dataset of human liver biopsy samples from five people with liver cirrhosis and five with a healthy liver. k , UMAP projection of all annotated CD45 + cell types and colored by human MSen Seurat score. l , UMAP projection of CD68 + cells (macrophages) in healthy and cirrhotic tissue samples. Color denotes human MSen Seurat score. m , Distribution of MSen Seurat score across resident macrophage (Kupffer cells) and non-resident monocyte and macrophage populations in healthy and cirrhotic conditions. P value represents the results of a two-sided t -test. n , Distribution of human MSen score across all CD45 + cell types in the dataset. ALD, alcohol-related liver disease; F, female; KC, Kupffer cell; IgA, immunoglobulin A; IL-C, innate lymphoid cell; M, male; MoMac, monocyte-derived macrophage; Mø, macrophage; PBC, primary biliary cholangitis; pDC, plasmacytoid dendritic cell. Panels a and j created in BioRender; Salladay-Perez, I. https://biorender.com/6umuckd (2026).

    Article Snippet: Mouse BMDM-derived and human PBMC-derived macrophage cultures were lysed with RNA STAT 60 (Amsbio) and ~50 ng per μl of RNA was submitted to the UCLA’s Technology Center for Genomics and Bioinformatics (TCGB) core for library preparation and 2 × 100 paired-end sequencing.

    Techniques: In Vitro, Irradiation, Derivative Assay, Gene Expression, Quantitative RT-PCR, Control, SDS Page, Immunostaining, Western Blot, Labeling, Staining, Flow Cytometry, RNA Sequencing, Single Cell